Human VEGF-C ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human VEGF-C ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of VEGF-C in human serum and plasma. An antibody specific for human VEGF-C was coated on a 96-well plate. Standards and samples are added to the wells and any VEGF-C present binds to the immobilized antibody. The wells are washed and biotinylated anti-human VEGF-C antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of VEGF-C present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area wound healing; tumor growth; vascular disease;

specific against

Human

detection target

vascular endothelial growth factor C( VEGF-C )
Background: Vascular endothelial growth factor C (VEGF-C) and VEGF-D constitute a VEGF subfamily that shares the conserved VEGF homology domain (VHD) with other VEGF family members, but is distinguished by their preferential formation of non-covalent homodimers. The main function of VEGF-C is in lymphangiogenesis, where it acts on lymphatic endothelial cells (LECs) primarily via its receptor VEGFR-3 promoting survival, growth and migration. It was shown to be a specific growth factor for lymphatic vessels in a variety of models. However, in addition to its effect on lymphatic vessels, it can also promote the growth of blood vessels and regulate their permeability. The effect on blood vessels can be mediated via its primary receptor VEGFR-3 or its secondary receptor VEGFR-2.

alias

VRP, Flt4-L

sensitivity

10 pg/ml

detection range

13.7-10000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P49767