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Human VE-Cadherin/CD144 PicoKine ELISA Kit

Product Code: BOS-EK1317

Verfügbarkeit: Auf Lager

Special Price 280,44 €

Regulärer Preis: 295,20 €

Zzgl. 19% USt.

Human VE-Cadherin/CD144 PicoKine ELISA Kit

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Beschreibung

Details

Human VE-Cadherin/CD144 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human VE-Cadherin in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human VE-Cadherin
Protein name: Cadherin-5
Gene Full Name: cadherin 5, type 2 (vascular endothelium)
abbreviation: CDH5
Background: Cadherin 5, type 2 or VE-cadherin also known as CD144, is encoded by the gene CDH5. It is a classical cadherin from the cadherin superfamily. This gene is mapped to mouse chromosome 8. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It has found that PECAM1, VE-cadherin and VEGFR2 together are sufficient to confer responsiveness to flow in heterologous cells.
UniProd ID:P33151
NCBI ID:1003

alias

7B 4|7B4|7B4 antigen|CADH5_HUMAN|Cadherin 5|Cadherin 5 type 2|Cadherin 5, type 2 (vascular endothelium)|Cadherin 5, type 2, VE cadherin (vascular epithelium)|cadherin, vascular endothelial|cadherin, vascular endothelial, 1|Cadherin-5|Cadherin5|CD 144|CD144|CD144 antigen|CDH 5|CDH5|CDH5 protein|Endothelial specific cadherin|FLJ17376|OTTHUMP00000174777|Vascular endothelial cadherin|Vascular epithelium cadherin|VE Cad|VE-cadherin|VEC|

Origin

Nativ and recombinant human VE-Cadherin

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, D48-Q599

sensitivity

<10pg/ml

detection range

312pg/ml-20000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|2.21|8.45|14.4|2.13|8.79|13.7
Standard deviation|0.095|0.313|0.691|0.124|0.466|1.04
CV(%)|4.3|3.7|4.8|5.8|5.3|7.6

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human VE-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VE-Cadherin has been precoated onto 96-well plates. Standards(NSO, D48-Q599) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VE-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human VE-Cadherin amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human VE-Cadherin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human VE-Cadherin standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|312|625|1250|2500|5000|10000|20000 O.D.|0.096|0.220|0.286|0.505|0.814|1.279|1.970|2.189

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human VE-Cadherin antibody|1
ST0000-20|Lyophilized recombinant human VE-Cadherin standard|20ng/tube�2
AR1107|Biotinylated anti- human VE-Cadherin antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller Boster
Herstellerland USA
Sensitivity <10pg/ml
Specific Against Human
Antigen Name Cadherin-5 (CDH5)
Detection Range 312pg/ml-20000pg/ml
Alias 7B 4, 7B4, 7B4 antigen, CADH5_HUMAN, Cadherin 5, Cadherin 5 type 2, Cadherin 5, type 2 (vascular endothelium), Cadherin 5, type 2, VE cadherin (vascular epithelium), cadherin, vascular endothelial, cadherin, vascular endothelial, 1, Cadherin-5, Cadherin5,
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Datasheet