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Human Ubiquitin+1 ELISA Kit

Product Code: BAS-3010303

Verfügbarkeit: Auf Lager

Human Ubiquitin+1 ELISA Kit

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Beschreibung

Details

Human Ubiquitin+1 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human Ubiquitin+1 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Ubiquitin+1 in Human serum, plasma and cell culture media. An antibody specific for Human Ubiquitin+1 was coated on a 96-well plate. Standards and samples are added to the wells and any Ubiquitin+1 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human Ubiquitin+1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Ubiquitin+1 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Apoptosis;

specific against

Human

detection target

ribosomal protein S27a(Ubiquitin+1)
Background: Ubiquitin is ubiquitously expressed in all eukaryotic organisms.. In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting.

alias

CEP80, HEL112, S27A, UBA80, UBC, UBCEP1, UBCEP80

sensitivity

15 pg/ml

detection range

27.4-20000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P62979

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller BioAim Scientific
Herstellerland Kanada
Sensitivity 15 pg/ml
Specific Against Human
Antigen Name ribosomal protein S27a
Detection Range 27.4-20000 pg/ml
Alias CEP80, HEL112, S27A, UBA80, UBC, UBCEP1, UBCEP80
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