BioAim Scientific (Kanada)
The Bioaim Human TSLP ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of TSLP in Human serum, plasma and cell culture media. An antibody specific for Human TSLP was coated on a 96-well plate. Standards and samples are added to the wells and any TSLP present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human TSLP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of TSLP present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Cell growth; proliferation;
thymic stromal lymphopoietin(TSLP) Background: Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM– pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of myeloid (CD11c+) dendritic cells.
Serum, plasma, Cell culture supernatant
|Antigen Name||thymic stromal lymphopoietin|
|Detection Range||3.3-800 pg/ml|