Human Trappin -2 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human Trappin-2 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Trappin-2 in human serum and plasma. An antibody specific for human Trappin-2 was coated on a 96-well plate. Standards and samples are added to the wells and any Trappin-2 present binds to the immobilized antibody. The wells are washed and biotinylated anti-human Trappin-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Trappin-2 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Inflammation;

specific against

Human

detection target

peptidase inhibitor 3( Trappin -2 )
Background: Trappin-2 is a member of the trappin family that includes the SLPI (secretory leukocyte protease inhibitor) protein. Elafin or ESI (elastase-specific inhibitor) and SKALP (skin-derived anti-leukoproteinase) are alternative names for trappin-2 and reflect its protease targets. Elafin has been found to have utility in serving as a biomarker for graft versus host disease of the skin. Elafin plays some role in gut inflammation.

alias

ESI, SKALP, WAP3, WFDC14, cementoin

sensitivity

4 pg/ml

detection range

6.9-5000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P19957