Human TRAIL R4 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human TRAIL R4 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of TRAIL R4 in Human serum, plasma and cell culture media. An antibody specific for Human TRAIL R4 was coated on a 96-well plate. Standards and samples are added to the wells and any TRAIL R4 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human TRAIL R4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of TRAIL R4 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Apoptosis;

specific against

Human

detection target

TNF receptor superfamily member 10d(TRAIL R4)
Background: Decoy receptor 2 (DCR2), also known as TRAIL receptor 4 (TRAILR4) and tumor necrosis factor receptor superfamily member 10D (TNFRSF10D), is a human cell surface receptor of the TNF-receptor superfamily. TRAIL binds at least five different receptors: TRAIL R1 (DR4), R2 (DR5), R3 (DcR1), R4 (DcR2), and osteoprotegerin (OPG). TRAIL receptors all exhibit cysteine-rich repeats characteristic of the TNF receptor superfamily. TRAIL R1 and R2 are type 1 transmembrane proteins with intracellular death domains (DD) and, subsequently, have the ability to mediate TRAIL-induced apoptosis. The remaining receptors are decoys that may regulate TRAIL activity by competing for binding sites with TRAIL R1 and R2. TRAIL R3 is membrane-associated via GPI linkage. TRAIL R4 is a type 1 transmembrane receptor, but exhibits a truncated cytoplasmic domain. Like the active receptors TRAIL R1 and R2, OPG has a DD motif but is a soluble protein.

alias

CD264, DCR2, TRAIL-R4, TRAILR4, TRUNDD

sensitivity

3 pg/ml

detection range

5.5-4000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q9UBN6