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Human TPO ELISA Kit

Product Code: BAS-3010143

Verfügbarkeit: Auf Lager

Human TPO ELISA Kit

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Beschreibung

Details

Human TPO ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human TPO ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of TPO in human serum and plasma. An antibody specific for human TPO was coated on a 96-well plate. Standards and samples are added to the wells and any TPO present binds to the immobilized antibody. The wells are washed and biotinylated anti-human TPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of TPO present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area megakaryocytopoiesis and thrombopoiesis;

specific against

Human

detection target

thyroid peroxidase( TPO )
Background: Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders. Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors. Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation. Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling.

alias

MSA, TDH2A, TPX

sensitivity

45 pg/ml

detection range

93.8-6000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P07202

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller BioAim Scientific
Herstellerland Kanada
Sensitivity 45 pg/ml
Specific Against Human
Antigen Name thyroid peroxidase
Detection Range 93.8-6000 pg/ml
Alias MSA, TDH2A, TPX
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