Human TNF-alpha/TNFA/TNFSF2 ELISA Kit

Brand

Sino Biological

short description

For the quantitative determination of Human TNF-alpha/TNFA/TNFSF2 concentration in cell culture supernates.

spezie

Human

natural samples' content determination

Human peripheral blood mononuclear cells ?2 x 10E6 cells/mL? were cultured in DMEM supplemented with 5% bovine calf serum, 50 µM ?-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate, stimulated with 1 µg/mL PHA for 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of natural TNFa, and measured 1144.57 pg/mL.

Cross Reaction

This assay recognizes both recombinant and natural Human TNFa. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

detection target

TNF
Antigene: 10602-HNAE
capture antibody type: mouse mAbdetection antibody type

sensitivity

11.96 pg/ml

detection range

23.44-1500 pg/ml

certificate

ISO9001-2008

pack size

96 Wells

storage and Stability

Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA

Principle of the Elisa

Solid Phase Sandwich ELISA

Kit Content

1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution