For the quantitative determination of TIMP-1 / TIMP1 concentration in serum and saliva.
The average concentration of Human TIMP1 in 10 normal human serum is 131.79 +/- 28.86 ng/mL ranging from 87.24 to 172.97 ng/mL and in 5 normal salvia is 363.10 +/- 131.44 ng/mL ranging from 239.42 to 550.67 ng/mL.
This assay recognizes both recombinant and natural Human TIMP1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. Cross-reactivity was observed to be 0.086% with 50 ng/mL of recombinant human MMP9.
TIMP1Antigene: 10934-H08Hcapture antibody type: mouse mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||7.8-500 pg/ml|
|Alias||TIMP-1 / TIMP1|