Human TGF beta1 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human TGF-beta1 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of TGF-beta1 in human serum and plasma. An antibody specific for human TGF-beta1 was coated on a 96-well plate. Standards and samples are added to the wells and any TGF-beta1 present binds to the immobilized antibody. The wells are washed and biotinylated anti-human TGF-beta1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of TGF-beta1 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Cancer Biomarker;

specific against

Human

detection target

transforming growth factor beta 1( TGF β 1 )
Background: Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. TGF-β is a multifunctional set of peptides that controls proliferation, differentiation, and other functions in many cell types. TGF-β acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGF-β activation and signaling may result in apoptosis. Many cells synthesize TGF-β and almost all of them have specific receptors for this peptide. TGF-⓵, TGF-⓶, and TGF-⓷ all function through the same receptor signaling systems. TGF-⓵ was first identified in human platelets as a protein with a molecular mass of 25 kilodaltons with a potential role in wound healing. It was later characterized as a large protein precursor (containing 390 amino acids) that was proteolytically processed to produce a mature peptide of 112 amino acids.TGF-⓵ plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGF-⓵.

alias

CED, DPD1, LAP, TGFB, TGFbeta

sensitivity

40 pg/ml

detection range

0.082-60 ng/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P01137