Human TGF beta RIII ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human TGF-beta RIII ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of TGF-beta RIII in Human serum, plasma and cell culture media. An antibody specific for Human TGF-beta RIII was coated on a 96-well plate. Standards and samples are added to the wells and any TGF-beta RIII present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human TGF-beta RIII antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of TGF-beta RIII present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis;

specific against

Human

detection target

transforming growth factor beta receptor 3(TGF β RIII)
Background: Most cell types express three sizes of receptors for TGF-beta. These are designated Type I, Type II and Type III. The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-beta1 and TGF-beta 3 with high affinity and TGF-beta 2 with much lower affinity. The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-beta. The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43 amino acid residue cytoplasmic domain.

alias

BGCAN, betaglycan

sensitivity

28 pg/ml

detection range

40.9-10000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q03167