BioAim Scientific (Kanada)
The Bioaim Human TFPI ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of TFPI in Human serum, plasma and cell culture media. An antibody specific for Human TFPI was coated on a 96-well plate. Standards and samples are added to the wells and any TFPI present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human TFPI antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of TFPI present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Coagulation ;
tissue factor pathway inhibitor (TFPI) Background: Tissue factor pathway inhibitor (or TFPI) is a single-chain polypeptide which can reversibly inhibit Factor Xa. It is a secreted protein with an N-terminal acidic region, three Kunitz (K) domains separated by two linker regions, and a C-terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF). The second K domain inhibits factor Xa. The third K domain binds to heparin. The C-terminal basic region may have several functions. Human TFPI-2 is structurally similar to TFPI and is downregulated in several cancers.
EPI, LACI, TFI1, TFPI
Serum, plasma, Cell culture supernatant
|Antigen Name||tissue factor pathway inhibitor|
|Detection Range||7.4-1800 pg/ml|
|Alias||EPI, LACI, TFI1, TFPI|