BioAim Scientific (Kanada)
The Bioaim Human SP-D ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of SP-D in Human serum, plasma and cell culture media. An antibody specific for Human SP-D was coated on a 96-well plate. Standards and samples are added to the wells and any SP-D present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human SP-D antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of SP-D present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Immune response;
surfactant protein D (SP-D) Background: Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. Surfactant Protein D (SP-D) is a collectin family protein consisting of a collagen-like sequence followed by a C-type lectin domain. It is synthesized primarily in the lung by alveolar type II cells and unciliated bronchial epithelial cells. In addition, SP-D is produced by epithelial cells of the gastrointestinal tract and exocrine glands. SP-D binds to patterns of neutral sugars on micro-organisms to mediate direct opsonization and phagocytosis and is an important component of the innate immune system.
COLEC7, PSP-D, SFTP4, SP-D
Serum, plasma, Cell culture supernatant
|Antigen Name||surfactant protein D|
|Detection Range||0.12-30 ng/ml|
|Alias||COLEC7, PSP-D, SFTP4, SP-D|