Human SLAM/CD150 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human SLAM in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human SLAM
Protein name: Signaling lymphocytic activation molecule
Gene Full Name: signaling lymphocytic activation molecule family member 1
abbreviation: SLAMF1
Background: Signaling lymphocytic activation molecule is a protein that in humans is encoded by the SLAMF1 gene. It belongs to the immunoglobulin gene superfamily. This gene is mapped to 1q23.3. It has found that SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes and a proportion of B cells, and is rapidly induced on naive T cells after activation. In MV-resistant cell lines, infection with clinical MV and expression of SLAM, but not CD46, caused cytopathic effects (CPE). The expression of SLAM on activated B and T lymphocytes correlates with the pathology of MV infection in humans and monkeys, in which lymphoid organs are the chief sites of MV replication and the binding of MV to SLAM may impair the signaling functions of SLAM in lymphocyte activation and inhibit Th0/Th1 cytokine production, thereby promoting Th2 cytokine production. It has reported that antibody-mediated ligation of SLAM on thymocytes triggered a protein tyrosine phosphorylation signal in T cells in a SAP-dependent manner. This signal also involved SHIP, the adaptor molecules DOK2, DOK1, and SHC and RASGAP.
ID:Q13291
ID:6504

alias

4933415F16|AA177906|CD 150|Cd150|CD150 antigen|Cdw150|Estm51|Ipo 3|IPO-3|Ipo3|MGC151472|MGC151476|OTTHUMP00000025670|OTTHUMP00000060252|RGD1560634|Signaling lymphocytic activation molecule|Signaling lymphocytic activation molecule family member 1|Signaling lymphocytic activation molecule family member 1, isoform CRA_a|Signaling lymphocytic activation molecule family member 1, isoform CRA_b|Signaling lymphocytic activation molecule precursor|Slaf1|SLAF1_HUMAN|SLAM family, member 1|Slamf 1|Slamf1|SLAMF1 protein

Origin

Nativ and recombinant human SLAM

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, A21-K236

sensitivity

<10pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1281|3355|5633|1426|3865|6134
Standard deviation|87.11|164.4|310|108.4|201|392.6
CV(%)|6.8|4.9|5.5|7.6|5.2|6.4

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human SLAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SLAM has been precoated onto 96-well plates. Standards(NSO, A21-K236) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SLAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SLAM amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human SLAM standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human SLAM standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.008|0.050|0.099|0.208|0.401|0.794|1.409|2.265

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human SLAM antibody|1
ST0000-10|Lyophilized recombinant human SLAM standard|10ng/tube�2
AR1107|Biotinylated anti- human SLAM antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4