Human Siglec-7/CD328 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human Siglec-7 in cell culture supernates, serum and plasma(heparin).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human Siglec-7
Protein name: Sialic acid-binding Ig-like lectin 7
Gene Full Name: sialic acid binding Ig like lectin 7
abbreviation: SIGLEC7
Background: Sialic acid-binding Ig-like lectin 7, also known as CD328 (cluster of differentiation 328) or AIRM1, is a protein that in humans is encoded by the SIGLEC7 gene. And this gene is mapped to chromosome 19. AIRM1 is a novel member of the sialoadhesin family characterized by 3 immunoglobulin-like extracellular domains (1 N-terminal V type and 2 C2 type) and a classic immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic portion. The highest amino acid sequence similarity found was with the myeloid-specific CD33 molecule, and similar to other sialoadhesin molecules, AIRM1 appears to mediate sialic acid-dependent ligand recognition.
ID:Q9Y286
ID:27036

alias

AIRM1 | AIRM-1 | AIRM 1 | CD328 | D siglec | D-siglec | HLDA 8 | p75 | QA79 | Siglec-7 ? SIGLEC7 ? SIGLEC19P ? SIGLECP2 | Q9Y286

Origin

Nativ and recombinant human Siglec-7

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, Q19-L353

sensitivity

<10pg/ml

detection range

125pg/ml-8000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|463|2258|4357|492|1709|4029
Standard deviation|25.46|155.66|309.3|31.98|126.46|358.58
CV(%)|5.5|6.9|7.1|6.5|7.4|8.9

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human Siglec-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Siglec-7 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q19-L353) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Siglec-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Siglec-7 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml human Siglec-7 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human Siglec-7 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|125|250|500|1000|2000|4000|8000 O.D.|0.009|0.099|0.175|0.302|0.613|1.105|1.812|2.287

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human Siglec-7 antibody|1
ST0000-10|Lyophilized recombinant human Siglec-7 standard|10ng/tube�2
AR1107|Biotinylated anti- human Siglec-7 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4