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BioAim Scientific (Kanada)
The Bioaim Human sICAM-1 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of sICAM-1 in human serum and plasma. An antibody specific for human sICAM-1 was coated on a 96-well plate. Standards and samples are added to the wells and any sICAM-1 present binds to the immobilized antibody. The wells are washed and biotinylated anti-human sICAM-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of sICAM-1 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Angiogenesis; cell adhesion; inflammation;
Human
intercellular adhesion molecule 1( sICAM-1 ) Background: ICAM-1 is a member of the immunoglobulin superfamily, the superfamily of proteins including antibodies and T-cell receptors. ICAM-1 is a transmembrane protein possessing an amino-terminus extracellular domain, a single transmembrane domain, and a carboxy-terminus cytoplasmic domain. The structure of ICAM-1 is characterized by heavy glycosylation, and the protein’s extracellular domain is composed of multiple loops created by disulfide bridges within the protein. It mediates the vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18).
BB2, CD54, P3.58
120 pg/ml
0.313-20 ng/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
Q99930
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | 120 pg/ml |
Specific Against | Human |
Antigen Name | intercellular adhesion molecule 1 |
Detection Range | 0.313-20 ng/ml |
Alias | BB2, CD54, P3.58 |