Human Serpin A4 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human Serpin A4 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Serpin A4 in Human serum, plasma and cell culture media. An antibody specific for Human Serpin A4 was coated on a 96-well plate. Standards and samples are added to the wells and any Serpin A4 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human Serpin A4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Serpin A4 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; Coagulation;

specific against

Human

detection target

serpin family A member 4(Serpin A4)
Background: The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

alias

KAL, KLST, KST, PI-4, PI4, kallistatin

sensitivity

120 pg/ml

detection range

0.143-35 ng/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P29622