BioAim Scientific (Kanada)
The Bioaim Human SDF-1 beta ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of SDF-1 beta in Human serum, plasma and cell culture media. An antibody specific for Human SDF-1 beta was coated on a 96-well plate. Standards and samples are added to the wells and any SDF-1 beta present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human SDF-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of SDF-1 beta present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Angiogenesis ; inflammation;
chemokine (C-X-C motif) ligand 12(SDF-1 β) Background: CXCL12, also known as SCYB12, PBSF and SDF-1 beta, is an 8.3 kDa, heparin-binding member of the CXC (or alpha-) family of chemokines. SDF-1 alpha and beta are reported to be monomers at neutral pH and physiologic ionic strength (4). SDF-1 alpha is also reported to form dimers in the presence of heparansulfate (8). On the cell surface, this may well facilitate SDF-1 interaction with its two receptors, CXCR4 and syndecan-4. Heparin sulfate is known to protect SDF-1 from proteolysis, and CXCR4 exists constitutively as a dimer. Among its many functions, CXCL12 is known to influence lymphopoiesis, regulate patterning and cell number of neural progenitors, and promote angiogenesis . It also enhances the survival of myeloid progenitor cells.
TPAR1, SDF1, TLSF, PBSF, IRH, SCYB12
Serum, plasma, Cell culture supernatant
|Antigen Name||chemokine (C-X-C motif) ligand 12|
|Detection Range||32.8-8000 pg/ml|
|Alias||TPAR1, SDF1, TLSF, PBSF, IRH, SCYB12|