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Human RRM2B / P53R2 ELISA Pair Set

Product Code: SEK13150

Verfügbarkeit: Auf Lager

Human RRM2B / P53R2 ELISA Pair Set

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Beschreibung

Details

Human RRM2B / P53R2 ELISA Pair Set

Brand

Sino Biological

short description

The Human RRM2B / P53R2 ELISA Pair Set is for the quantitative determination of Human RRM2B / P53R2. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for RRM2B / P53R2 coated on a 96-well plate. Standards and samples are added to the wells, and any RRM2B / P53R2 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-RRM2B / P53R2 monoclonal antibody is then added, producing an antibody-antigen-antibody モsandwichヤ. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of RRM2B / P53R2 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

spezie

Human

detection target

RRM2B
Standard (antigene): Each vial contains 63 ng of recombinant RRM2B. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended.
capture antibody type: 0.5 mg/mL of mouse anti-RRM2B monoclonal antibody. Dilute to a working concentration of 2 µg/mL in CBS before coating. (Catalog: # 13150-MM11)
detection antibody type: 0.5 mg/mL mouse anti-RRM2B monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 µg/mL in detection antibody dilution buffer before use. (Catalog: # 13150-MM04)
Lable: HRP

sensitivity

The minimum detectable dose of Human RRM2B / P53R2 was determined to be approximately 47 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

47 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.

Weitere Informationen

Weitere Informationen

Hersteller Sino Biological
Herstellerland China
Sensitivity The minimum detectable dose of Human RRM2B / P53R2 was determined to be approximately 47 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Specific Against Human
Antigen Name RRM2B
Detection Range 47 pg/ml
Zertifikat ISO9001-2008
Alias RRM2B / P53R2
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Datasheet