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BioAim Scientific (Kanada)
The Bioaim Human Resistin ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Resistin in human serum and plasma. An antibody specific for human Resistin was coated on a 96-well plate. Standards and samples are added to the wells and any Resistin present binds to the immobilized antibody. The wells are washed and biotinylated anti-human Resistin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Resistin present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Peptide Hormone; Lipid metabolism;
Human
Resistin ( Resistin ) Background: Resistin, also known ADSF and FIZZ3, is a secreted peptide hormone that circulates as either a noncovalent trimer, or a disulfide-linked homohexamer. Resistin is expressed by white adipocytes, splenocytes, astrocytes, and anterior pituitary epithelium. It blocks Insulin-stimulated uptake of glucose by adipocytes and promotes glucose release by hepatocytes. High circulating Insulin levels induce Resistin secretion which then antagonize Insulin action. Resistin increases the production of LDL in human liver cells and also degrades LDL receptors in the liver. As a result, the liver is less able to clear ‘bad’ cholesterol from the body. Resistin accelerates the accumulation of LDL in arteries, increasing the risk of heart disease.Resistin adversely impacts the effects of statins, the main cholesterol-reducing drug used in the treatment and prevention of cardiovascular disease.
ADSF, FIZZ31, RSTN, XCP1, RETN
< 1 pg/ml
1.6-400 pg/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
Q9HD89
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | < 1 pg/ml |
Specific Against | Human |
Antigen Name | Resistin |
Detection Range | 1.6-400 pg/ml |
Alias | ADSF, FIZZ31, RSTN, XCP1, RETN |