For the quantitative determination of Human REG1B concentration in serum.
The average concentration of Human REG1B in 10 normal human serum is 26.18 +/- 6.05 ng/mL ranging from 15.06 to 34.17 ng/mL.
This assay recognizes both recombinant and natural Human REG1B. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. Cross-reactivity was observed to be 94% with 0.04 ng/mL of recombinant REG1A.
REG1BAntigene: 11638-H08Hcapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||6.25-400 pg/ml|