BioAim Scientific (Kanada)
The Bioaim Human Progranulin ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Progranulin in Human serum, plasma and cell culture media. An antibody specific for Human Progranulin was coated on a 96-well plate. Standards and samples are added to the wells and any Progranulin present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human Progranulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Progranulin present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Cancer Biomarker;
granulin (Progranulin) Background: Progranulin, also known as proepithelin and PC cell-derived growth factor (PCDGF), is a secreted, glycosylated protein with multiple repeats of the granulin/epithelin motif. Mutations in the GRN gene have been implicated in up to 25% of frontotemporal lobar degeneration, inherited in an autosomal dominant fashion with high penetrance. Several loss-of-function mutations disease-causing mutations in GRN have been identified.
CLN11, GEP, GP88, PCDGF, PEPI, PGRN
Serum, plasma, Cell culture supernatant
|Detection Range||0.102-25 ng/ml|
|Alias||CLN11, GEP, GP88, PCDGF, PEPI, PGRN|