Human PLGF ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human PLGF ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of PLGF in human serum and plasma. An antibody specific for human PLGF was coated on a 96-well plate. Standards and samples are added to the wells and any PLGF present binds to the immobilized antibody. The wells are washed and biotinylated anti-human PLGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of PLGF present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis, metabolism, metastasis;

specific against

Human

detection target

placental growth factor ( PLGF )
Background: PLGF (placenta growth factor) is a homodimeric growth factor that competes with VEGF for binding to VEGF R1/Flt-1. It therefore increases the availability of VEGF to bind to VEGF R2/KDR/Flk-1 and trigger angiogenesis. It can form heterodimers with some forms of VEGF and decrease the angiogenic effect of VEGF on VEGF R2. Circulating PLGF levels increase during pregnancy, reaching a peak in mid-gestation; this increase is attenuated in preeclampsia. PLGF induces monocyte activation and migration as well as production of inflammatory cytokines and VEGF. These activities facilitate wound, bone fracture, and cardiac repair, but also contribute to inflammation in active sickle cell disease and atherosclerosis. PLGF can also inhibit TIMP3 expression in the spleen, leading to immune triggering of hypertension.

alias

D12S1900L, PIGF, PLGF,PlGF-2, SHGC-10760, PGF

sensitivity

< 1 pg/ml

detection range

1.4-1000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P49763