Human PECAM-1 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human PECAM-1 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of PECAM-1 in Human serum, plasma and cell culture media. An antibody specific for Human PECAM-1 was coated on a 96-well plate. Standards and samples are added to the wells and any PECAM-1 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human PECAM-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of PECAM-1 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; cell adhesion; inflammation;

specific against

Human

detection target

platelet and endothelial cell adhesion molecule 1 (PECAM-1)
Background: CD31/PECAM-1 is a heavily glycosylated transmembrane homophilic adhesion protein that is highly expressed on endothelial cells and at a lower level on platelets, granulocytes, macrophages, dendritic cells, T and B cells, and NK cells. It is required for the transepithelial migration of leukocytes. CD31 signaling increases the activation threshold of T cells and inhibits dendritic cell development. The extracellular domain of CD31 is released during endothelial cell apoptosis. This fragment circulates in the serum of patients suffering from myocardial infarction, acute ischaemic stroke, and multiple sclerosis, conditions that involve tissue damage and endothelial cell apoptosis.

alias

CD31, CD31/EndoCAM, GPIIA', PECA1,PECAM-1, endoCAM

sensitivity

4 pg/ml

detection range

8.2-6000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P16284