Human PECAM-1/CD31 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human PECAM-1 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human PECAM-1
Protein name: Platelet endothelial cell adhesion molecule
Gene Full Name: platelet/endothelial cell adhesion molecule 1f
abbreviation: PECAM1
Background: Human platelet/endothelial cell adhesion molecule-1(PECAM1), an important member of the immunoglobulin gene superfamily, is widely distributed on cells of the vascular system and mediates cellular interactions through both homophilic and heterophilic adhesive mechanisms. The function of PECAM1 in vitro has begun to be understood, but its function in vivo is yet to be established.1 The PECAM1 locus is on the long arm of chromosome 17, in the region q23-qter. To confirm this observation and obtain a more precise localization of the PECAM1 locus, fluorescence in situ hybridization was conducted. Together our data allowed assignment of the PECAM1 locus to the region 17q23.2 To examine the functional role of PECAM-1 in regulating platelet-collagen interactions, 2 different approaches were applied using recombinant human PECAM-1-immunoglobulin chimeras and platelets derived from PECAM-1-deficient mice.3 PECAM-1, an integral membrane protein with an essential role in TEM, is found in this compartment and constitutively recycles evenly along endothelial cell borders.4 The standard used in this kit is the product of gene recombinant expression(extracellular part), consisting of 574 amino acids(Q28-K601) with the molecular weight of 64.5KDa. After glycosylating, its molecular weight changes to 90-100KDa.
ID:P16284
ID:5175

alias

CD31|CD31 antigen|EndoCAM|GPIIA|HEC7|Leukocyte surface protein|PECA1|PECA1_HUMAN|PECAM 1|PECAM-1|Pecam1|Platelet endothelial cell adhesion molecule|Platelet/endothelial cell adhesion molecule

Origin

Nativ and recombinant human PECAM-1

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

CHO, Q28-K601

sensitivity

<10pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1282|2520|5023|1526|2768|5281
Standard deviation|87.2|120.96|261.2|116|182.7|338
CV(%)|6.8|4.8|5.2|7.6|6.6|6.4

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human PECAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PECAM-1 has been precoated onto 96-well plates. Standards(CHO, Q28-K601) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PECAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PECAM-1 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human PECAM-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human PECAM-1 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.086|0.176|0.284|0.400|0.696|1.145|1.629|2.207

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human PECAM-1 antibody|1
ST0000-10|Lyophilized recombinant human PECAM-1 standard|10ng/tube�2
AR1107|Biotinylated anti- human PECAM-1 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4