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Human PDK / PDZ binding kinase / TOPK ELISA Pair Set

Product Code: SEK10729

Verfügbarkeit: Auf Lager

Human PDK / PDZ binding kinase / TOPK ELISA Pair Set

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Beschreibung

Details

Human PDK / PDZ binding kinase / TOPK ELISA Pair Set

Brand

Sino Biological

short description

The human PBK / TOPK ELISA Pair Set is for the quantitative determinationof human PBK / TOPK. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is asolid phase sandwich ELISA (Enzyme-Linked ImmunosorbentAssay). It utilizes a monoclonal antibody specific for PBK / TOPK coated on a 96-well plate. Standards and samples are added tothe wells, and any PBK / TOPK present binds to the immobilizedantibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-PBK / TOPKmonoclonal antibody is thenadded, producing an antibody-antigen-antibody "sandwich". The wells are againwashed and TMB substrate solution is loaded, which produces color in proportionto the amount of PBK / TOPK present in the sample. To end theenzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

spezie

Human

detection target

PBK
Standard (antigene): Each vial contains 40 ng of recombinant PBK. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended.
capture antibody type: 0.3 mg/mL of mouse anti-PBK monoclonal antibody. Dilute to a working concentration of 2 µg/mL in CBS before coating. (Catalog: # 10729-MM10)
detection antibody type: 0.5 mg/mL mouse-anti-PBK monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 µg/mL in detection antibody dilution buffer before use. (Catalog: # 10729-MM05)
Lable: HRP

sensitivity

The minimum detectable dose of Human PBK / TOPK was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

62.5 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.

Weitere Informationen

Weitere Informationen

Hersteller Sino Biological
Herstellerland China
Sensitivity The minimum detectable dose of Human PBK / TOPK was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Specific Against Human
Antigen Name PDK
Detection Range 62.5 pg/ml
Zertifikat ISO9001-2008
Alias PDK / PDZ binding kinase / TOPK
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