Human PDGF-BB EasyTestTM ELISA kit

Brand

BioAim Scientific (Kanada)

short description

The BioAim Human PDGF-BB EasyTestTM ELISA kit can quantitatively measure PDGF-BB in human serum or plasma. It is a simple and rapid technology for the quantitation of antigen in a range of sample matrices. The whole process takes less than 1.5 hours with high accuracy and precision. EasyTestTM ELISA is faster and easier to perform than standard format ELISA with less reagent handling and fewer pipetting steps.
Research Area wound healing; tumor growth; vascular disease;

specific against

Human

detection target

platelet-derived growth factor beta polypeptide(PDGF-BB)
Background: The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta). Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene.

alias

IBGC5, PDGF-2, PDGF2, SIS, SSV, c-sis

sensitivity

<1 pg/ml

detection range

40.9-10000 pg/ml

sample type

Serum

pack size

1x96 Wells

Assay time

1.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P01127