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Boster
For quantitative detection of human OSM in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human OSMProtein name: Oncostatin-MGene Full Name: oncostatin Mabbreviation: OSMBackground: OSM(Oncostatin M) is a protein that in humans is encoded by the OSM gene. OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. The OSM gene is mapped on 22q12.2. Of these cytokines it most closely resembles leukemia inhibitory factor(LIF) in both structure and function. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR. ID:P13725 ID:5008
MGC20461|ONCM_HUMAN|Oncostatin M precursor|Oncostatin-M|OncostatinM|OSM|OTTMUSP00000005249|RP23-453B22.1
Nativ and recombinant human OSM
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
E.coli, A26R-220
<10pg/ml
15.6pg/ml-1000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|104|351|627|135|392|658 Standard deviation|5.72|16.15|42.64|8.37|20.78|47.4 CV(%)|5.5|4.6|6.8|6.2|5.3|7.2
20-25
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human OSM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for OSM has been precoated onto 96-well plates. Standards(E.coli, A26R-220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for OSM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human OSM amount of sample captured in plate.
Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human OSM standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human OSM standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.023|0.113|0.210|0.332|0.589|1.062|1.574|2.251
Catalog number|Description|Quantity-|96-well plate precoated with anti- human OSM antibody|1ST0000-10|Lyophilized recombinant human OSM standard|10ng/tubex2AR1107|Biotinylated anti- human OSM antibody|130ul(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Human |
Antigen Name | Oncostatin-M (OSM) |
Detection Range | 15.6pg/ml-1000pg/ml |
Alias | MGC20461, ONCM_HUMAN, Oncostatin M precursor, Oncostatin-M, OncostatinM, OSM, OTTMUSP00000005249, RP23-453B22.1 |