×

Registrierung

Profile Informationen

Login Daten

oder Einloggen

Vorname ist erforderlich!
Nachname ist erforderlich!
Vorname ist ungültig!
Nachname ist ungültig!
Dies ist keine E-Mail-Adresse!
E-Mail-Adresse ist erforderlich!
Dies E-Mail-Adresse ist bereit registriert!
Passwort ist erforderlich!
Geben Sie ein gültiges Passwort ein!
Bitte geben Sie 6 oder mehr Zeichen ein!
Bitte geben Sie 16 oder weniger Zeichen ein!
Passwörter sind nicht dasselbe!
Geschäftsbedingungen sind erforderlich!
E-Mail oder Passwort ist falsch!

Human OSM/Oncostatin M PicoKine ELISA Kit

Product Code: BOS-EK0478

Verfügbarkeit: Auf Lager

Human OSM/Oncostatin M PicoKine ELISA Kit

Bewerten Sie dieses Produkt als Erster

OR

Fragen zur Lieferzeit

Angebot anfordern

Datasheet anfordern

Beschreibung

Details

Human OSM/Oncostatin M PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human OSM in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human OSM
Protein name: Oncostatin-M
Gene Full Name: oncostatin M
abbreviation: OSM
Background: OSM(Oncostatin M) is a protein that in humans is encoded by the OSM gene. OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. The OSM gene is mapped on 22q12.2. Of these cytokines it most closely resembles leukemia inhibitory factor(LIF) in both structure and function. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR.
UniProd ID:P13725
NCBI ID:5008

alias

MGC20461|ONCM_HUMAN|Oncostatin M precursor|Oncostatin-M|OncostatinM|OSM|OTTMUSP00000005249|RP23-453B22.1

Origin

Nativ and recombinant human OSM

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

E.coli, A26R-220

sensitivity

<10pg/ml

detection range

15.6pg/ml-1000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|104|351|627|135|392|658
Standard deviation|5.72|16.15|42.64|8.37|20.78|47.4
CV(%)|5.5|4.6|6.8|6.2|5.3|7.2

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human OSM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for OSM has been precoated onto 96-well plates. Standards(E.coli, A26R-220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for OSM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human OSM amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human OSM standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human OSM standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.023|0.113|0.210|0.332|0.589|1.062|1.574|2.251

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human OSM antibody|1
ST0000-10|Lyophilized recombinant human OSM standard|10ng/tubex2
AR1107|Biotinylated anti- human OSM antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller Boster
Herstellerland USA
Sensitivity <10pg/ml
Specific Against Human
Antigen Name Oncostatin-M (OSM)
Detection Range 15.6pg/ml-1000pg/ml
Alias MGC20461, ONCM_HUMAN, Oncostatin M precursor, Oncostatin-M, OncostatinM, OSM, OTTMUSP00000005249, RP23-453B22.1
Bewertungen
Tags

Produkttags

Verwenden Sie Leerzeichen um Tags zu trennen. Verwenden Sie Apostrophe (') für Phrasen.

Datasheet