Human NT-4 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human NT-4 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of NT-4 in Human serum, plasma and cell culture media. An antibody specific for Human NT-4 was coated on a 96-well plate. Standards and samples are added to the wells and any NT-4 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human NT-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of NT-4 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Neuronal differentiation and survival;

specific against

Human

detection target

Neurotrophin-4(NT-4)
Background: Neurotrophin-3 (NT-3) and Neurotrophin-4 (NT-4) are members of the NGF family of neurotrophic factors that are required for the differentiation and survival of specific neuronal subpopulations. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate active propeptides and mature protein homodimers. Mature NT-4 also forms heterodimers with BDNF or NT-3. Neurotrophins induce dimerization and activation of Trk family receptors and promote the development and survival of selected peripheral and CNS neurons. All neurotrophins have six conserved disulfide-linked cysteine residues. NT-4 is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase.

alias

GLC10, GLC1O, NT-4, NT-4/5, NT-5, NT4, NT5, NTF5

sensitivity

< 1 pg/ml

detection range

1.4-1000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P34130