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BioAim Scientific (Kanada)
The Bioaim Human NOV ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of NOV in Human serum, plasma and cell culture media. An antibody specific for Human NOV was coated on a 96-well plate. Standards and samples are added to the wells and any NOV present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human NOV antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of NOV present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Angiogenesis; Aging; angiogenesis, cell survival, and immune response;
Human
nephroblastoma overexpressed (NOV) Background: NOV belongs to the CCN (CTGF/CYR61/NOV) family of secreted proteins that share a common multimodular organization. Each protein contains an IGFBP domain, a von Willebrand factor type C domain, a thrombospondin type I domain and a cysteine knot domain. NOV interacts with several proteins and is involved in both internal and external cell signaling.
CCN3, IBP-9, IGFBP-9, IGFBP9h, NOV
20 pg/ml
0.041-30 ng/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
P48745
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | 20 pg/ml |
Specific Against | Human |
Antigen Name | nephroblastoma overexpressed |
Detection Range | 0.041-30 ng/ml |
Alias | CCN3, IBP-9, IGFBP-9, IGFBP9h, NOV |