Human NOTCH-1 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human NOTCH-1 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of NOTCH-1 in Human serum, plasma and cell culture media. An antibody specific for Human NOTCH-1 was coated on a 96-well plate. Standards and samples are added to the wells and any NOTCH-1 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human NOTCH-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of NOTCH-1 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Embryogenesis and morphogenesis;

specific against

Human

detection target

Neurogenic locus notch homolog protein 1(NOTCH-1)
Background: Notch proteins (so named for ‘notches’ in fly wings) are type I transmembrane glycoproteins involved in specifying cell fates and defining boundaries between different cell types during developmental processes. Notch extracellular domains are large, having 34-36 EGF-like repeats followed by three notch/Lin-12 repeats. Notch proteins interact with transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand binding, a series of cleavage events results in the release of the Notch intracellular domain (NICD), which translocates to the nucleus and initiates transcription of Notch-responsive genes. Thus, Notch acts as both a ligand-binding receptor and a nuclear factor that regulates transcription.The four mammalian Notch receptors appear to have distinct functions, since they do not compensate for one another in genetic studies. Mutations in Notch receptors also lead to specific developmental disorders.

alias

AOS5, AOVD1, TAN1, hN1

sensitivity

15 pg/ml

detection range

28.7-7000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P46531