BioAim Scientific (Kanada)
The Bioaim Human Nidogen-1 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Nidogen-1 in Human serum, plasma and cell culture media. An antibody specific for Human Nidogen-1 was coated on a 96-well plate. Standards and samples are added to the wells and any Nidogen-1 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human Nidogen-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Nidogen-1 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Angiogenesis; cell adhesion;Apoptosis;
Nidogen-1(Nidogen-1) Background: Both nidogen-1 and nidogen-2 are essential components of the basement membrane alongside other components such as type IV collagen, proteoglycans (heparan sulfate and glycosaminoglycans), laminin and fibronectin. Nidogen-1 is a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes. Structurally it (along with perlecan) connects the networks formed by collagens and laminins to each other. It may also play a role in cell interactions with the extracellular matrix.
Serum, plasma, Cell culture supernatant
|Detection Range||0.12-30 ng/ml|