Human NAP-2 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human NAP-2 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of NAP-2 in human serum and plasma. An antibody specific for human NAP-2 was coated on a 96-well plate. Standards and samples are added to the wells and any NAP-2 present binds to the immobilized antibody. The wells are washed and biotinylated anti-human NAP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of NAP-2 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; inflammation;

specific against

Human

detection target

nucleosome assembly protein 1 like 4( NAP-2 )
Background: Neutrophil activating peptide 2 (NAP-2), connective tissue activating protein III (CTAP-III) and beta-thrombogulin (beta-TG), are proteolytically processed carboxy-terminal fragments of platelet basic protein (PBP), which is found in the alpha-granules of human platelets. NAP-2 is a member of the CXC chemokines and is named CXCL7. Similar to other ELR domain containing CXC chemokines, such as IL-8 and the GRO proteins, NAP-2 binds CXCR2 and chemoattracts and activates neutrophils. It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator.

alias

NAP1L4b, NAP2, NAP2L, hNAP2

sensitivity

2 pg/ml

detection range

4.1-1000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q99733