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BioAim Scientific (Kanada)
The Bioaim Human MSP ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of MSP in Human serum, plasma and cell culture media. An antibody specific for Human MSP was coated on a 96-well plate. Standards and samples are added to the wells and any MSP present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human MSP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of MSP present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Inflammation;
Human
macrophage stimulating 1(MSP) Background: Macrophage stimulating protein (MSP), also known as HGF-like protein, and scatter factor-2, is a member of the HGF family of growth factors. MSP is secreted as an inactive single chain precursor (pro-MSP) that contains a PAN/APPLE-like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity. Pro-MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF-4 or RAR, respectively. Circulating pro-MSP is proteolytically cleaved in response to tissue injury to yield a biologically active disulfide linked heterodimer consisting of a 45 – 62 kDa alpha and a 25 – 35 kDa beta chain. MSP induces macrophage and keratinocyte proliferation and osteoclast activation. It also inhibits LPS- or IFN-induced iNOS and IL-12 expression by macrophages, and prevents apoptosis of epithelial cells separated from the ECM.
D3F15S2, DNF15S2, HGFL, MSP, NF15S2
4 pg/ml
2.7-2000 pg/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
P26927
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | 4 pg/ml |
Specific Against | Human |
Antigen Name | macrophage stimulating 1 |
Detection Range | 2.7-2000 pg/ml |
Alias | D3F15S2, DNF15S2, HGFL, MSP, NF15S2 |