BioAim Scientific (Kanada)
The Bioaim Human MMR ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of MMR in Human serum, plasma and cell culture media. An antibody specific for Human MMR was coated on a 96-well plate. Standards and samples are added to the wells and any MMR present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human MMR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of MMR present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Immune response;
mannose receptor, C type 1 (MMR) Background: he human Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 190 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells. The human MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1371 aa extracellular region, a 21 aa transmembrane segment and a 46 aa cytoplasmic domain. The cysteine-rich domain mediates recognition of sulfated N‑acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin. Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine. The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin‑coated vesicles. MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation.
CD206, CLEC13D, CLEC13DL, MMRL1, bA541I19.1, hMR, MRC1
Serum, plasma, Cell culture supernatant
|Antigen Name||mannose receptor, C type 1|
|Detection Range||0.41-100 ng/ml|
|Alias||CD206, CLEC13D, CLEC13DL, MMRL1, bA541I19.1, hMR, MRC1|