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BioAim Scientific (Kanada)
The Bioaim Human MMP-8 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of MMP-8 in Human serum, plasma and cell culture media. An antibody specific for Human MMP-8 was coated on a 96-well plate. Standards and samples are added to the wells and any MMP-8 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human MMP-8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of MMP-8 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Angiogenesis; Cancer Biomarker;
Human
Matrix metalloproteinase-8(MMP-8) Background: The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
CLG1, HNC, MMP-8, PMNL-CL
4 pg/ml
8.2-6000 pg/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
P22894
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | 4 pg/ml |
Specific Against | Human |
Antigen Name | Matrix metalloproteinase-8 |
Detection Range | 8.2-6000 pg/ml |
Alias | CLG1, HNC, MMP-8, PMNL-CL |