Human MIP-1 delta ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human MIP-1 delta ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of MIP-1 delta in Human serum, plasma and cell culture media. An antibody specific for Human MIP-1 delta was coated on a 96-well plate. Standards and samples are added to the wells and any MIP-1 delta present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human MIP-1 delta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of MIP-1 delta present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; inflammation;

specific against

Human

detection target

C-C motif chemokine ligand 15(MIP-1 delta)
Background: Macrophage inflammatory protein 1 delta (MIP-1 delta), also named Leukotactin-1 (LKN-1), MIP-5, HCC-2, and NCC-3, together with mouse C10, mouse MIP-1 gamma and human MPIF-1, constitute a subgroup of CC chemokines which contain six instead of four conserved cysteine residues. The two additional cysteine residues in MIP-1 delta/LKN-1 form a third disulfide bond. CCL15 is expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3.

alias

LKN1; NCC3; SY15; HCC-2; LKN-1; MIP-5; NCC-3; SCYL3; MIP-1D; MRP-2B; SCYA15; HMRP-2B; MIP-1 delta

sensitivity

1 pg/ml

detection range

4.1-1000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q16663