Human MIF ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human MIF ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of MIF in human serum and plasma. An antibody specific for human MIF was coated on a 96-well plate. Standards and samples are added to the wells and any MIF present binds to the immobilized antibody. The wells are washed and biotinylated anti-human MIF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of MIF present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Cancer Biomarker; inflammation; metabolic regulation;

specific against

Human

detection target

macrophage migration inhibitory factor ( MIF )
Background: MIF (macrophage migration inhibitory factor) is a pro-inflammatory cytokine that is released during microbial infection, ARDS, rheumatoid arthritis, atherosclerosis, type 2 diabetes, and cancer. Bacterial antigens stimulate white blood cells to release MIF into the blood stream. The circulating MIF binds to CD74 on other immune cells to trigger an acute immune response. Hence, MIF is classified as an inflammatory cytokine. Furthermore, glucocorticoids also stimulate white blood cells to release MIF and hence MIF partially counteracts the inhibitory effects. Finally trauma activates the anterior pituitary gland to release MIF. It signals through CXCR4, CXCR7, or complexes of CD74-CD44.

alias

GIF, GLIF, MMIF

sensitivity

5 pg/ml

detection range

8.2-6000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P14174