BioAim Scientific (Kanada)
The Bioaim Human MICA ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of MICA in human serum and plasma. An antibody specific for human MICA was coated on a 96-well plate. Standards and samples are added to the wells and any MICA present binds to the immobilized antibody. The wells are washed and biotinylated anti-human MICA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of MICA present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area viral infection;
MHC class I polypeptide-related sequence A( MICA ) Background: MHC class I chain-related genes MICA and MICB are transmembrane glycoproteins that function as ligands for human NKG2D. Recognition of MICA or MICB by NKG2D results in the activation of NK cell cytolytic activity and/or cytokine production. MICA/B are minimally expressed on normal cells, but are frequently expressed on or shed from epithelial tumors and can be induced by bacterial and viral infections. MICA and MICB recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.
Serum, plasma, Cell culture supernatant
|Antigen Name||MHC class I polypeptide-related sequence A|
|Detection Range||13.7-10000 pg/ml|