Human Mesothelin ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human Mesothelin ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Mesothelin in Human serum, plasma and cell culture media. An antibody specific for Human Mesothelin was coated on a 96-well plate. Standards and samples are added to the wells and any Mesothelin present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human Mesothelin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Mesothelin present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Cancer Biomarker;

specific against

Human

detection target

Mesothelin(Mesothelin)
Background: Mesothelin, also known as CAK1 and ERC, is derived from a precursor that also includes Megakaryocyte Potentiating Factor (MPF). Following cleavage of the precursor, Mesothelin remains attached to the cell surface via a GPI linkage. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is upregulated in mesotheliomas and a range of carcinomas and adenomas, including mesothelioma and ovarian and pancreatic adenocarcinoma.

alias

MPF, SMRP

sensitivity

90 pg/ml

detection range

0.12-30 ng/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q13421