Human MBL ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human MBL ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of MBL in Human serum, plasma and cell culture media. An antibody specific for Human MBL was coated on a 96-well plate. Standards and samples are added to the wells and any MBL present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human MBL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of MBL present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Immune response;

specific against

Human

detection target

mannose binding lectin 2(MBL)
Background: Mannan binding lectin (MBL) belongs to the collectin family of innate immune defense proteins, which bind an array of carbohydrate patterns on pathogen surfaces. Collectin family members share several common structural features including a N-terminal, cysteine-rich domain, a collagen-like region, an alpha-helical coiled-coil neck domain and a C-terminal, C-type (Ca2+-dependent ) lectin or carbohydrate recognition domain. MBL homotrimerizes to form a structural unit joined by N-terminal disulfide bonds. These homotrimers further associate into oligomeric structures of up to six units. MBL belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host.

alias

MBL, HSMBPC, MBP1, COLEC1, MBL2D, MBP-C, MBP, MBPD

sensitivity

15 pg/ml

detection range

0.034-25 ng/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P11226