Human Lymphotactin ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human Lymphotactin ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Lymphotactin in Human serum, plasma and cell culture media. An antibody specific for Human Lymphotactin was coated on a 96-well plate. Standards and samples are added to the wells and any Lymphotactin present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human Lymphotactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Lymphotactin present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Inflammation;

specific against

Human

detection target

X-C motif chemokine ligand 1 (Lymphotactin)
Background: Lymphotactin (Lptn), also known as SCM-1a and ATAC, belongs to the C or gamma subfamily of chemokines and has been designated XCL1. Chemokine (C motif) ligand (XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. Cellular sources for XCL1 include activated thymic and peripheral blood CD8+ T cells. This chemokine attracts T cells. In humans, XCL1 is closely related to another chemokine called XCL2, whose gene is found at the same locus on chromosome 1. XCL1 induces it chemotactic function by binding to a chemokine receptor called XCR1.

alias

ATAC, LPTN, LTN, SCM-1, SCM-1a, SCM1, SCM1A, SCYC1

sensitivity

50 pg/ml

detection range

0.069-50 ng/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P47992