Human LOX-1 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human LOX-1 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of LOX-1 in Human serum, plasma and cell culture media. An antibody specific for Human LOX-1 was coated on a 96-well plate. Standards and samples are added to the wells and any LOX-1 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human LOX-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of LOX-1 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Immune response; Lipid metabolism;

specific against

Human

detection target

oxidized low density lipoprotein receptor 1(LOX-1)
Background: Lectin-like Oxidized Low-density Lipoprotein Receptor 1 (LOX-1), also known as Oxidized Low-density Lipoprotein Receptor 1 (OLR1), is a type II transmembrane receptor belonging to the class E scavenger receptor (SR-E) subfamily of the C-type lectin family. It binds and supports the internalization of multiple structurally unrelated macromolecules. It exists on the cell surface as covalent homodimers, which can further associate into non-covalently-linked oligomers. Cell surface LOX-1 can also be cleaved to release the soluble LOX-1 extracellular domain.Its gene is regulated through the cyclic AMP signaling pathway. The protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor.

alias

CLEC8A, LOX1, LOXIN, SCARE1, SLOX1

sensitivity

< 1 pg/ml

detection range

2.1-500 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P78380