The Human Leptin / LEP ELISA Pair Set is for the quantitative determination of Human Leptin / LEP. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human Leptin / LEP coated on a 96-well plate. Standards and samples are added to the wells, and any Human Leptin / LEP present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human Leptin / LEP monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human Leptin / LEP present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
LEPStandard (antigene): Each vial contains 172 ng of recombinant Human Leptin / LEP. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 500 pg/mL is recommended. capture antibody type: 0.5 mg/mL of mouse anti-Human Leptin / LEP monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 µg/mL in PBS before coating. (Catalog: # 10221-MM03)detection antibody type: 0.2 mg/mL of mouse anti-Human Leptin / LEP monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4°C;). Dilute to working concentration of 1 µg/mL in detection antibody dilution buffer before use. (Catalog: # 10221-MM05)Lable: HRP
The minimum detectable dose of Human Leptin / LEP was determined to be approximately 7.81 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of Human Leptin / LEP was determined to be approximately 7.81 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|Detection Range||7.81 pg/ml|
|Alias||Leptin / LEP|