For the quantitative determination of Human LAIR2 (CD306) concentration in cell lysate.
The concentration of Human LAIR2 detected in Raw264.7 and MCF7 cell lysates were respectively 529.31 pg/mL and 461.52 pg/mL.The total protein concentration of Raw264.7 and MCF7 cell lysates were about 9.3 mg/mL and 6.7 mg/mL.
This assay recognizes both recombinant and natural Human LAIR2. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
LAIR2Antigene: 10868-H08Hcapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Antigen Name||LAIR2 (CD306)|
|Detection Range||7.8-500 pg/ml|