For the quantitative determination of JAML / AMICA1 concentration in cell lysate.
The concentration of Human JAML detected in a Jurkat cell lysate was 1641.5 pg/mL.The Jurkat cell lysate' total protein concentration was about 6.0 mg/mL .
This assay recognizes both recombinant and natural Human JAML. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
AMICA1Antigene: 10120-H08Hcapture antibody type: mouse mAbdetection antibody type
CEACAM1 / CD66a
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||312.5-20000 pg/ml|
|Alias||JAML / AMICA1|