For the quantitative determination of Human Interferon Gamma / IFN gamma / IFNG concentration in cell culture supernates.
Human peripheral blood mononuclear cells ?2 x 10E6 cells/mL?were cultured in were cultured in1640 supplemented with 10% bovine calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate, stimulated with 1 µg Anti-CD3 for 4days. Aliquots of the culture supernates were removed and assayed for levels of natural ?-IFN, and measured 56.44 ng/mL.
This assay recognizes both recombinant and natural Human ?-IFN. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IFNGAntigene: 11725-HNAScapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Antigen Name||Interferon Gamma|
|Detection Range||93.75-6000 pg/ml|
|Alias||Interferon Gamma / IFN gamma / IFNG|