For the quantitative determination of Human IL6 / IL-6 concentration in cell culture supernates.
Human foreskin fibroblast cells (1 x 10E5 cells/mL) were cultured in DMEM supplemented with 10% fetal bovine serum, stimulated for 2 days with 50 ng/mL IL17A. Aliquots of the culture supernates were removed and assayed for levels of natural IL6, and measured 1045.17 pg/mL.
This assay recognizes both recombinant and natural Human IL6. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IL6Antigene: 10395-HNAEcapture antibody type: mouse mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||18.75-1200 pg/ml|
|Alias||IL6 / IL-6|