For the quantitative determination of Human IL32 concentration in serum.
Ten serum samples from apparently healthy people were evaluated for the presence of IL32a in this assay.The average concentration of four detected samples is 0.96ng/mL. Other samples measured less than the lowest IL32a standard, 62.5 pg/mL.
This assay recognizes both recombinant and natural human IL32a. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IL32Antigene: 11064-H08Hcapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||62.5-4000 pg/ml|