The human IL1R2 (IL1RB / CD121b) ELISA Pair Set is for the quantitative determinationof human IL1R2. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL1R2(IL1RB / CD121b) coated on a 96-well plate. Standards andsamples are added to the wells, and any IL1R2 ( IL1RB / CD121b) present binds tothe immobilized antibody. The wells are washed and a horseradish peroxidaseconjugated mouse anti-IL1R2 monoclonal antibody is then added, producing anantibody-antigen-antibody "sandwich". The wells are again washed and TMBsubstrate solution is loaded, which produces color in proportion to the amountof IL1R2 present in the sample. To end the enzyme reaction, the stop solutionis added and absorbances of the microwell are read at 450 nm.
IL1R2Standard (antigene): Each vial contains 24 ng of recombinant IL1R2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 600 pg/mL is recommended. capture antibody type: 0.5 mg/mL of rabbit anti-IL1R2 monoclonal antibody. Dilute to a working concentration of 2.0 µg/mL in CBS before coating (Catalog: # 10111-R041)detection antibody type: 0.5 mg/mL mouse anti-IL1R2 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 µg/mL in detection antibody dilution buffer before use. (Catalog: # 10111-MM02)Lable: HRP
The minimum detectable dose of human IL1R2 (IL1RB / CD121b) was determined to be approximately 9.375 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of human IL1R2 (IL1RB / CD121b) was determined to be approximately 9.375 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard|
|Detection Range||9.375 pg/ml|
|Alias||IL1R2 / IL1RB / CD121b|